This 4 day course is intended to train university staff, postgraduate students and biomedical scientists in the understanding and application of transmission electron microscopy (TEM) in the biomedical sciences.

Participants are trained in methods of sample processing, embedding, cutting and staining?(using their own samples if available), the principles and operation of the TEM and the presentation of images (including electronic presentation). In addition, participants will acquire the skills to analyse and present data, and be aware of the role, importance and relevance of TEM in biological research.

The course is accredited by the Institute of Biomedical Sciences (27 points) and is limited to 8 students per course to ensure individual attention and equipment access.

Topics covered:

Lectures:

• Health & Safety
• Choice of fixatives
• Cell structure and resolution
• TEM design
• Cryosectioning and immunolabelling for TEM
• Special techniques
• Data presentation

Total Lecture Time: 6.5 hrs

Practicals

• Fixation
• Processing and embedding
• Glass knife making
• Ultamicrotomy
• Staining grids
• TEM operation / Sample viewing and imaging
• EM Image Quiz

Total Practical Time: 21 hrs

For more information on course content and timetable, please use the download links below.

Course Dates and Booking

The course runs approximately once every 6 months and is often over subscribed. To register for a place on the next available course please contact Patricia Goggin ( p.goggin@soton.ac.uk , SGH x4815).